Phylogenomics of Cypriniformes, the most diverse order of freshwater fishes: consensus, challenges and limitations
Cypriniformes, the most species-rich order of freshwater fishes (∼5,000 species), represents a key lineage for understanding vertebrate diversification in freshwater ecosystems. This clade includes several highly miniaturized and understudied lineages whose phylogenetic placements have long remained contentious. Here, we present the first phylogenomic analysis of Cypriniformes with complete family-level representation and broad genus-level coverage, encompassing 316 species comprising approximately 30% of all described genera. Our dataset integrates 257 newly assembled genomes with publicly available resources and analyzes multiple sets of genome-wide markers using both concatenation-based and coalescent-aware approaches. The general concordance among analytical frameworks indicates that the backbone topology of Cypriniformes is now established, allowing clear identification of well-supported clades and regions of persistent conflict. Our results strengthen the evolutionary relationships of several miniaturized lineages, while identifying recalcitrant relationships shaped by both biological processes and model artefacts that can yield superficially similar patterns of phylogenetic conflict. Our study substantially expands genomic representation and establishes a phylogenomic foundation for future comparative, developmental, and evolutionary research in this freshwater radiation.
Authors
- V. K. Anoop (ORCID: https://orcid.org/0000-0003-4500-4972)
- Hiranya Sudasinghe (ORCID: https://orcid.org/0000-0003-0994-3462)
- Catherine L. Peichel (ORCID: https://orcid.org/0000-0002-7731-8944)
- Michael Matschiner (ORCID: https://orcid.org/0000-0003-4741-3884)
- Neelesh Dahanukar (ORCID: https://orcid.org/0000-0001-7162-9023)
- Lukas Rüber (ORCID: https://orcid.org/0000-0003-0125-008X)
- Kerstin Howe (ORCID: https://orcid.org/0000-0003-2237-513X)
- Rajeev Raghavan (ORCID: https://orcid.org/0000-0002-0610-261X)
- Maurice Kottelat (ORCID: https://orcid.org/0000-0003-3638-2824)
- Richard Michael Durbin (ORCID: https://orcid.org/0000-0002-9130-1006)
- Ralf Britz (ORCID: https://orcid.org/0000-0002-0126-4660)
- Walter Salzburger (ORCID: https://orcid.org/0000-0002-9988-1674)
- Heok Hui Tan (ORCID: https://orcid.org/0000-0003-2227-8866)
- Joerg Bohlen (ORCID: https://orcid.org/0000-0002-7068-2321)
- Kevin Conway
Institutions
- University of Bern (CH)
- National University of Singapore (SG)
- University of Basel (CH)
- Kerala University of Fisheries and Ocean Studies (IN)
- University of Cambridge (GB)
- Shiv Nadar University (IN)
- Wellcome Sanger Institute (GB)
- Natural History Museum of Bern (CH)
- Czech Academy of Sciences, Institute of Animal Physiology and Genetics (CZ)
- Senckenberg Natural History Collections Dresden (DE)
- Ludwig-Maximilians-Universität München (DE)
- Texas A&M University (US)
Publication Details
- Journal
- Molecular Biology and Evolution
- Published
- 2026-10-26
- DOI
- https://doi.org/10.1093/molbev/msag261
- Citations
- 8
- Primary Topic
- Fish Biology and Ecology Studies
- Type
- article
- Field-Weighted Citation Impact
- 49.14
Funders
- University of Montana
- University of Bern
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung