A synthetic biology toolkit for rationally designing genetic circuits in Acinetobacter baumannii
By enabling tunable transcriptional control through the characterized promoters and ensuring the ability to downregulate gene expression via CRISPRi, this synthetic biology toolkit lays the foundation for the rational design of genetic circuits to study and counteract AMR in <i>A. baumannii</i>. The modular platform here characterized provides a valuable resource for the iGEM community to advance functional genomic approaches against this alarming global health challenge.
Authors
- Massimo Bellato (ORCID: https://orcid.org/0000-0001-7428-0656)
- Chiara Zanin
- Cristiano De Pittà (ORCID: https://orcid.org/0000-0001-8013-8162)
- Francesca Galiazzo
- Claudia Del Vecchio (ORCID: https://orcid.org/0000-0001-5645-9126)
- Alex D. Martini
- Sara Letrari
- Luca Guarnieri
- Valentina Del Giudice
- Ignazio Castagliuolo
- Ilgin Ertan
- Livio Trainotti
- Chiara Ravazzolo
- Stefano Intini
- Giorgia D’angelo
- Marco Costanzo
- Asia Picchi
- Tommaso Varaschin
- Lisa Faccincani
- Niccolò Venturini Degli Esposti
Institutions
- University of Padua (IT)
Publication Details
- Journal
- Frontiers in Systems Biology
- Published
- 2026-01-15
- DOI
- https://doi.org/10.3389/fsysb.2025.1668595
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fondazione Cassa di Risparmio di Padova e Rovigo
- Università degli Studi Roma Tre
- Università degli Studi di Padova
- Università degli Studi di Pavia