Interdisciplinary Tools to Safeguard and Amplify Aquatic Genetic Resource Use: A Foundation for Industrial-Scale Quality Control for Fertilization

Genetic resources are becoming increasingly important in aquatic species, especially in sectors such as aquaculture and biomedical research. These advancements, however, lack standardized methodology to consistently improve efficient use of gametes for fertilization and to eliminate male variation during spawning. This study provides a conceptual basis for generalizable quality control in artificial spawning of aquatic species by using interdisciplinary, industrial-scale tools to calculate a fertilization unit (e.g., the amount of sperm required to reliably fertilize the eggs produced by a female). Blue catfish (<i>Ictalurus furcatus</i>), zebrafish (<i>Danio rerio</i>), and eastern oysters (<i>Crassostrea virginica</i>) were used as diverse representative species. Comparisons among aquatic species were reviewed, fertilization units were defined, and a sensitivity analysis was performed to assess how deviations from the fertilization unit could affect artificial spawning efficiency. Overall, reproductive strategy (e.g., gamete biology) and production setting significantly influenced the fertilization unit. Employing a fertilization unit decreased "wasted" sperm and reduced male variability during spawning. Furthermore, fertilization efficiency dropped significantly when sperm use strayed from the fertilization unit, declining with both underuse and overuse, especially in oysters and catfish. Standardizing gamete use in aquatic species is essential for economic planning and achieving commercial-scale production, especially when investing in selectively bred or cryopreserved sperm.