Improved Dye-Filling Protocol for Multiple Nematode Species

Dye-filling has been used extensively to study the development and morphology of sensory neurons in the nematode <i>C. elegans</i> . Here, we report a modified dye-filling protocol that improves the robustness of amphid, phasmid, and inner labial neuron dye-filling in <i>C. elegans</i> , as well as in the nematode satellite model <i>P. pacificus.</i> Our method also allows for the consistent visualization of male specific ray sensory neurons and associated support cells in these species.