High-affinity optimization potential of the virus neutralizing antibody with twin cysteine-stabilized complementarity-determining region 3
These findings establish that conformational stabilization of the H3 CDR in seed antibodies is a critical determinant for successful AI-driven affinity maturation. The study proposes a strategic framework for antibody development that prioritizes structurally stabilized H3 CDR regions, offering a robust approach to generating high-potency therapeutics against rapidly evolving viral pathogens.
Authors
- Zhongpeng Zhao (ORCID: https://orcid.org/0000-0002-9147-6252)
- Dongsheng Zhao (ORCID: https://orcid.org/0000-0003-2279-9928)
- Zhang Zhang (ORCID: https://orcid.org/0000-0001-6088-2293)
- Dan-Dan Zeng
- Jing Li
- Gang Dong
- Guang-Yu Zhao
- Tao Jiang
- Qi Yin
- Yue Zhang
- Fan Tong
- Sen Zhang
Institutions
- Shanxi Agricultural University (CN)
- Military Medical Academy (RS)
- Academy of Military Medical Sciences (CN)
- Military Medical Academy (EG)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2026-01-14
- DOI
- https://doi.org/10.3389/fcimb.2025.1693895
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Key Research and Development Program of China