Genomic and Transcriptomic Insights into the Evolution and Parasitic Strategy of the Woody-Plant Nematode <i>Pratylenchus vulnus</i>

The root-lesion nematode <i>Pratylenchus vulnus</i> parasitizes a wide range of hosts, including woody perennials such as walnut (<i>Juglans regia</i>) and grapevine (<i>Vitis vinifera</i>), significantly damaging roots and reducing yields. Here, we present a high-quality, chromosome-level genome assembly of <i>P</i>. <i>vulnus</i> (61.7 Mb across six chromosomes). Comparative genomic analysis revealed high collinearity in protein-coding genes between <i>P</i>. <i>vulnus</i> and the root-knot nematode included in this analysis, <i>Meloidogyne graminicola</i>, indicating a close evolutionary relationship between these genera of plant parasites. Large chromosomal regions in <i>P. vulnus</i> lack synteny with other nematode genomes, have comparatively low GC content (<30%), and are enriched in genes with unique or lineage-specific functions. Transcriptome analysis highlighted dynamic, stage-specific expression of genes involved in parasitism, development, and metabolism. Additionally, we identified an extensive repertoire of putative effector genes and characterized lineage-specific expansions of cell wall-degrading enzyme families. Overall, these findings provide insight into the genome organization, chromosome evolution, and parasitism-related gene repertoire in a woody plant-parasitizing nematode. [Formula: see text] Copyright © 2026 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.