RPA-CRISPR/Cas12a-based detection of Pasteurella multocida: establishment and initial application
The method showed a detection limit of 5 × 10<sup>-1</sup> copies/μL, and the reactions were completed within 30 min. When applied to 102 clinical samples, the RPA-CRISPR/Cas12a assay yielded a positive rate of 40.20% (41/102), which was 4.1 times higher than that of PCR. This assay offers a promising tool for rapid and instrument-free detection of Pm in frontline clinical settings.
Authors
- Huijun Shi (ORCID: https://orcid.org/0000-0001-6090-3699)
- Yuefeng Chu (ORCID: https://orcid.org/0000-0002-9443-2677)
- Quanxin Wu
- Rulong Chen
- Na Li (ORCID: https://orcid.org/0000-0003-3619-7899)
- Chaoqun Yan (ORCID: https://orcid.org/0000-0002-2025-5655)
- Xiaozhen Li
- Qiang Fu
- Li Yang
- Xuelian Ma
- Youquan Zhuang
Institutions
- Xinjiang Agricultural University (CN)
- Xinjiang Production and Construction Corps (CN)
- Animal Husbandry & Veterinary (IN)
- Lanzhou Veterinary Research Institute (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Frontiers in Veterinary Science
- Published
- 2026-01-14
- DOI
- https://doi.org/10.3389/fvets.2025.1730229
- Primary Topic
- Microbial infections and disease research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Xinjiang Agricultural University
- Modern Agricultural Technology Industry System of Shandong province