Bioprinting collagenase-responsive hydrogel for controlled release of cowpea mosaic virus immunotherapy
In this work, we developed a collagenase-responsive hydrogel system to covalently load cancer immunotherapy candidate cowpea mosaic virus (CPMV) using 3D digital light processing bioprinting technology. CPMV was functionalized with norbornene groups (CPMV-NB), which was then bioprinted into hydrogels with 8-arm polyethylene glycol norbornene and a collagenase-cleavable peptide via photoinduced thiol-ene click chemistry. This strategy enabled stable retention of CPMV-NB within the hydrogels and achieved controlled release of CPMV-NB triggered by collagenase. Furthermore, released CPMV-NB retained its immunogenicity to stimulate immune cells.
Authors
- Zhongchao Zhao (ORCID: https://orcid.org/0000-0002-3736-6677)
- Jacob Schimelman (ORCID: https://orcid.org/0000-0002-9028-2632)
- Zhuohong Wu (ORCID: https://orcid.org/0000-0002-5318-9092)
- Steven Fiering (ORCID: https://orcid.org/0000-0003-0624-974X)
- Nicole Steinmetz
- Yazhi Sun
- Shaochen Chen
- Yi Xiang
Institutions
- Dartmouth College (US)
- University of California San Diego (US)
- La Jolla Bioengineering Institute (US)
- University of California, Davis (US)
Publication Details
- Journal
- Biofabrication
- Published
- 2026-01-13
- DOI
- https://doi.org/10.1088/1758-5090/ae37de
- Citations
- 1
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 21.28
Funders
- National Science Foundation
- National Institutes of Health
- University of California, San Diego
- National Cancer Institute