A hybrid combination of in vitro cultured buccal mucosal cells using two different methodologies, complementing each other in successfully repairing a stricture-inflicted human male urethral epithelium
This study, a first of its kind, clarifies the rationale and advantages of combining 3D Festigel-expanded epithelial cells with the paracrine effect of IGF-1-secreting 2D fibroblast-like cells in a single transplantation strategy, thereby explaining the successful clinical outcomes reported in BEES-HAUS. Further research on this hybrid cell combination is recommended to expand this approach for regenerating and repairing other tissues and organs.
Authors
- Vaddi Surya Prakash
- Koji Ichiyama
- Senthilkumar Preethy (ORCID: https://orcid.org/0000-0002-9333-0361)
- Yoshine Mayumi (ORCID: https://orcid.org/0000-0003-1741-8385)
- Shojiro Katoh (ORCID: https://orcid.org/0000-0002-8355-9074)
- Masaru Iwasaki (ORCID: https://orcid.org/0000-0003-3766-8744)
- Yusuke Hirano
- Akio Horiguchi
- Kenichiro Ojima
- Samuel J. K. Abraham
- Toshihiro Kushibiki
- Masayuki Shinchi
- Rajappa Senthilkumar
Institutions
- Academy of Medical Sciences (GB)
- St. Mary Medical Center (US)
- DiscoveryBioMed (United States) (US)
- GC Corporation (Japan) (JP)
- Regenerative Medicine Institute (MX)
- Edogawa Hospital (JP)
- National Defence Medical Centre (CA)
- Tissue Dynamics (Israel) (IL)
- Kidney Centre (PK)
- University of Yamanashi (JP)
Publication Details
- Journal
- Frontiers in Urology
- Published
- 2026-01-14
- DOI
- https://doi.org/10.3389/fruro.2025.1720445
- Primary Topic
- Tissue Engineering and Regenerative Medicine
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- ESRC National Centre for Research Methods, University of Southampton