Integrative Taxonomy to Assess the Parasitoid Complex of the Jumping Plant-Louse Cacopsylla pulchella (Hemiptera: Psyllidae) on Cercis siliquastrum in Central and Southern Italy

Urban green spaces host complex arthropod communities, in which natural insect antagonists play a key role in regulating pest populations. The jumping plant-louse <i>Cacopsylla pulchella</i> is a sap-sucking pest widespread across Europe that attacks <i>Cercis siliquastrum</i> L., which is commonly used as an ornamental tree. Heavy infestations may contribute to host tree decline and cause indirect damage in urban environments by reducing aesthetic value and by extensive deposition of honeydew secretions on surrounding surfaces. As with many phytophagous insects occurring in urban contexts, information on the natural enemies of this species remains limited, particularly in Italy, and requires further documentation. Here, we investigated the parasitoids associated with <i>C. pulchella</i> in central and southern Italy based on surveys conducted between 2022 and 2025. Specimens were obtained from infested plant material and identified using an integrative taxonomic approach combining detailed morphological examination with DNA barcoding. <i>Prionomitus mitratus</i> was confirmed as the primary parasitoid of <i>C. pulchella</i>, while two species, <i>Pachyneuron muscarum</i> and <i>Pachyneuron aphidis</i>, were identified as hyperparasitoids. In addition, a single specimen of <i>Anastatus bifasciatus</i> was also recorded emerging from the psyllid as a hyperparasitoid. Molecular analyses generated the first publicly available mitochondrial and nuclear sequences for <i>P. mitratus</i>. For <i>Pachyneuron</i>, molecular results showed variable correspondence with available reference sequences, reflecting the uneven representation of species-level data for <i>Pteromalidae</i> in public databases. By integrating morphological and molecular evidence, this study clarifies trophic relationships within the <i>C. pulchella</i> parasitoid complex. It provides vouchered molecular references to support future taxonomic and ecological research in urban ecosystems.