Comparative flow cytometry immunophenotyping of ASCs expanded in conventional flasks versus automated bioreactors
The expansion method significantly influences the phenotypic composition of ASCs. HFB systems offer a promising alternative for large-scale ASC manufacturing by better maintaining subpopulation diversity. These findings emphasize the need for functional validation of ASC subtypes and careful consideration of expansion platforms in clinical-grade cell production.
Authors
- Guoqiang Ren (ORCID: https://orcid.org/0000-0001-9126-3890)
- Morten Brøndum Sørensen (ORCID: https://orcid.org/0009-0008-1644-1835)
- Zongzhe Xuan
- Sara Aghazadeh (ORCID: https://orcid.org/0000-0002-4824-1757)
- Qiuyue Peng
- Hiva Alipour
- Fereshteh Dardmeh
- Simone Riis Porsborg
- Trine Fink
Institutions
- Ningbo Medical Center Lihuili Hospital (CN)
- Aalborg University (DK)
Publication Details
- Journal
- Frontiers in Cell and Developmental Biology
- Published
- 2026-01-13
- DOI
- https://doi.org/10.3389/fcell.2026.1748921
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Aalborg Universitet