Comparative assessment of the broth microdilution and VITEK 2 systems for antifungal susceptibility testing of Candida auris (Candidozyma auris) Isolates

In conclusion, VITEK 2 showed a high level of agreement with the BMD method in the echinocandin susceptibility test of C. auris, while very high ME and VME ratios were observed for amphotericin B and fluconazole. These findings indicate that VITEK 2 amphotericin B and fluconazole AFST results should be interpreted carefully and validated using reference methods. Furthermore, the differences between CDC and EUCAST breakpoint interpretations, particularly for amphotericin B, highlight the significant impact of breakpoint selection on antifungal susceptibility categorization in C. auris.