Technical Considerations for Blood RNA Sequencing in Genetic Testing: Evaluation of Globin Depletion Methods, Batch Effects, and Sample Types
Globin-RR is effective, reproducible, and practical for use in clinical whole-blood RNA-seq. With high concordance and a slightly broader gene detection range than whole blood, PBMCs are a viable alternative sample type for routine genetic diagnostic tests.
Authors
- Yongguo Yu (ORCID: https://orcid.org/0000-0002-4472-0910)
- Yanjie Fan (ORCID: https://orcid.org/0000-0001-7006-733X)
- Xiaoyan Huo (ORCID: https://orcid.org/0009-0002-2219-3571)
- Jie Wang (ORCID: https://orcid.org/0000-0002-3638-5446)
- Ting Xu (ORCID: https://orcid.org/0000-0002-1381-1609)
- Qianfeng Zhao
- Huili Liu (ORCID: https://orcid.org/0000-0002-5535-5015)
- Yumeng Ma
- Xinyi Lu
- Yi Liu
- Xiaomei Luo
Institutions
- XinHua Hospital (CN)
Publication Details
- Journal
- Annals of Laboratory Medicine
- Published
- 2026-01-13
- DOI
- https://doi.org/10.3343/alm.2025.0434
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00