Acute Toxicity of Caffeine in Humans

This study addresses the need for a quantitative assessment of the median lethal dose (LD50) of caffeine in humans following oral administration. The aim was to estimate the human oral LD50 by extrapolating data obtained from laboratory animals, including mice, rats, guinea pigs, rabbits, dogs, and cats. Extrapolation was performed using three approaches: the method of Yu.R. Rybolovlev and R.S. Rybolovlev, the guidelines of the U.S. Environmental Protection Agency (EPA), and the method of D.B. Laurence and A.L. Bacharach. The estimated human oral LD50 of caffeine and its confidence interval are presented.