Automated workflow for genotyping individual transposon library variants

This automated workflow enables reliable genotyping while reducing hands-on time and minimizing human error. Its modular, standardized design and validated protocols promote protocol reuse and sharing while broadening access to open-source laboratory automation. While the throughput of the workflow is limited by the deck capacity of the liquid handler, it can be scaled by subsequent runs or parallel processing on multiple liquid handlers. In addition, the workflow can be further streamlined through integration of an OT-2 adapted colony picker. Overall, this open-source workflow provides a robust foundation for scalable and reproducible genotyping of individual variants in genome engineering and synthetic biology applications.