In vitro Propagation of Ancestral Tomato Plant Using 6-Benzyladenine and α-Naphthaleneacetic Acid

The aim of this study was to ensure the rapid reproduction of ancestral tomatoes under sterile conditions for the continuation of their generation. In vitro, clonal propagation of tomato plants was investigated on medium containing different concentrations of the hormones 6-Benzyl Adenine (BA) and α-Naphthalene Acetic acid (NAA) in explants. Hypocotyl, cotyledon and cotyledon nodes explants were taken from 20-day-old tomato seedlings and their combinations containing BA and NAA were cultured on Murashige Skoog (MS) medium. Subsequently, all explants were transferred to hormone-free MS medium. As a result of the study, the cotyledon explant showed the greatest increase in callus diameter at a concentration of 0.25 BA + 0.4 NAA ppm. This concentration was followed by a two-fold increase at 0.50 BA + 0.8 NAA ppm concentration. The number of shoots in the callus was observed significantly in the cotyledon explant and was highest at 0.25 BA + 0.2 NAA ppm concentration. Significant results were found in hypocotyl explants regarding callus formation and development (callus number (5/5)). Explants taken from the cotyledonary node were observed to be planted more in pots. Acclimatization and fruit formation were highest in explants taken from the cotyledonary node of the ancestor tomato.