DNA and RNA-based next-generation sequencing for companion diagnostic rearrangement detection in solid tumors

A higher rate of CDx RE detection-most significantly for NRG1 and NTRK fusions-was observed using concurrent DNA-NGS and RNA-NGS compared to DNA-NGS alone. Our results highlight the complementary nature of these methods. Given the substantial clinical benefit of RE-targeted therapies, an integrated DNA/RNA profiling strategy should be part of routine clinical care.