IMMUNOHISTOCHEMICAL ASSESSMENT OF CD20 EXPRESSION IN RAT KIDNEYS 1 HOUR AFTER ADMINISTRATION OF LEIURUS MACROCTENUS SCORPION VENOM

The aim of the work: to assess the degree of CD20 expression in the kidneys of rats 1 hour after the administration of the venom of the scorpion Leiurus macroctenus. Materials and Methods. The study used 10 white male laboratory rats weighing 200 g (±10 g). The venom of scorpions from the Buthidae family, specifically the genus Leiurus and the species Leiurus macroctenus, was administered to rats intramuscularly (0.5 ml of a previously dissolved venom solution in saline; 28.8 μg/ml; LD50 = 0.08 mg/kg). To identify the subpopulation of CD20 cells in kidney tissue, rabbit recombinant primary antibody Anti-CD20 (ab64088, Abcam, USA) was used. Results. In the kidney parenchyma of control rats, the CD20 precipitate was not detected. Immunohistochemical staining is negative in all morphological structures, particularly in the renal corpuscles, nephron tubules, and interstitium. After 1 hour of the experiment, following the administration of scorpion Leiurus macroctenus venom, immunohistochemical studies of the kidney revealed single CD20 cells, which are rarely found, scattered mainly in the peritubular interstitium without a tendency to group. Conclusions. One hour after the administration of Leiurus macroctenus venom to rats, a low level of CD20 expression was observed in the kidney tissue of animals with single B lymphocytes in the peritubular interstitium, indicating an early stage of the humoral response.