Identification and susceptibility profile of microorganisms isolated directly from urine in a cross-sectional study using matrix-assisted laser desorption/ionization time-of-flight and Phoenix M50

The antimicrobial susceptibility method varies depending on the species-antibiotic combination; therefore, specific studies for each species are crucial. Despite these challenges, the direct method offers significant advantages in diagnostic speed and emphasize its potential for improving clinical decision-making, though further validation and protocol refinement are needed, particularly for Gram-positive pathogens.