Acceptability of screening for hepatitis C virus in healthcare workers

Abstract Background : Healthcare workers (HCWs) face occupational exposure to hepatitis C virus (HCV). Screening acceptability is essential to implement new strategies. Aim : To assess HCV screening acceptability and associated factors among HCWs in a Romanian multidisciplinary hospital. Methods : Cross-sectional study within the “Cancer Prevention at Work” project (2024–2025). Out of 1,189 invited HCWs, 910 (76.5%) participated; 712 accepted blood testing (Group A), 198 completed questionnaires (Group B), and 279 refused entirely (Group C). Anti-HCV antibodies were detected using a combined ELISA and chemiluminescence test; positives underwent real-time RT-PCR test. Chi-square and Fisher tests analysed associations (p<0.05). Results : Women were significantly more likely to accept testing than men (p=0.021). Younger age groups participated more (p<0.001); participants ≥61 years refused most often. Stretcher bearers had the lowest acceptance; administrative staff the highest (p=0.022 for Group A vs B). Lower education correlated with refusal (p=0.044). Smokers accepted testing more than non-smokers (p=0.043). No significant associations with residence, comorbidities, or HBV vaccination. Of 712 tested, 10 (1.4%) were anti-HCV antibody positive; 1 (0.14%) had PCR positive with active infection. Conclusion : HCV screening acceptability was high (76.5%) but varied by sex, age, profession, education, and smoking status. Targeted interventions for older, lower-educated, and high-exposure groups (e.g., stretcher bearers) are needed to support occupational HCV elimination.