Enhanced detection of dermal-binding autoantibodies in pemphigoid diseases with a modified indirect immunofluorescence cell-based mosaic assay using IgG4 and optimized testing conditions: a single-centre retrospective study
Patzelt et al. previously characterized dermal-binding autoantibodies using an indirect immunofluorescence (IIF) mosaic assay with cells transfected with p200-laminin β4, laminin-332 or collagen VII subunits among specimens showing a dermal pattern on IIF using salt-split skin (IIF-SSS) and IgG. This study expanded testing to include specimens with either a dermal or mixed pattern on IIF-SSS and demonstrated improved test sensitivity with modified testing methods.
Authors
- Shruti Agrawal (ORCID: https://orcid.org/0000-0002-1620-9429)
- Julia Scott Lehman (ORCID: https://orcid.org/0000-0002-7389-3853)
- Colleen R. Lange
- Carilyn N. Wieland (ORCID: https://orcid.org/0000-0002-7706-370X)
- Amer N. Kalaaji
- M. J. Camilleri
- Emma F Johnson
- C. Miller
- Carly J Shore
- Anatilde M Gonzalez Guerrico
- Nneka I Comfere
Institutions
- Mayo Clinic in Arizona (US)
Publication Details
- Journal
- British Journal of Dermatology
- Published
- 2026-01-09
- DOI
- https://doi.org/10.1093/bjd/ljag007
- Primary Topic
- Autoimmune Bullous Skin Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00