Farming Model Design and Evaluation Biological Activity of Vermiwash Worm (Eudrilus Eugeniae)

An automated vermiwash farming system was developed to regulate soil moisture and facilitate efficient vermiwash collection. The system's performance was evaluated under both stable and disturbed conditions, maintaining soil moisture setpoints at 63 % and 66 %, respectively. Results demonstrated the system’s reliable responsiveness and stability, confirming its feasibility for automated vermiwash production. Chemical analysis of the vermiwash indicated the following compositions: organic carbon at 12.56 %, total organic matter at 21.00 %, total nitrogen at 2.61 %, and total phosphorus at 2.07 %. Biochemical assays further revealed the presence of peptides (0.64 ± 0.04 mg/mL) and proteins (0.44 ± 0.00 mg/mL). Enzymatic activities measured in the vermiwash included amylase (0.039 ± 0.001 U/mL), cellulase (0.0013 ± 0.0001 U/mL), and protease (0.486 ± 0.044 U/mL). The antioxidant potential peaked on day 45, with a maximum scavenging activity of 47.97 ± 0.21 %. However, no antimicrobial activity was detected against the tested microorganisms. These findings highlight the system's potential for sustainable vermiwash production with beneficial biochemical properties, though further exploration is needed for its antimicrobial applications.