COVID-19 Vaccination Knowledge, Attitudes, Perception, and Practices Among Frontline Healthcare Workers in Tunisia, 2024

<b>Background/Objectives</b>: Healthcare workers (HCW) in primary care settings play a significant role in recommending vaccines to patients. We aimed to describe COVID-19 vaccination knowledge, attitudes, perception, and practices (KAPP) of HCWs in Tunisia and identify associated factors. <b>Methods</b>: We conducted a national cross-sectional survey (29 January to 3 February 2024) among HCWs in primary public healthcare centers using purposive sampling. Factors associated with good knowledge, positive attitude, and good practice, measured through Likert scales using face-to-face questionnaires, were identified using binary logistic regression. <b>Results</b>: We included 906 HCWs (mean age = 41.87 ± 8.89 years). In total, 37.75% (342/906) of HCWs had good knowledge and perception, 4.30% (39/906) had a positive attitude, and 24.9% (226/906) had good practices related to COVID-19 vaccination. Working in urban compared to rural areas was associated with good knowledge (aOR = 1.57, 95%CI = 1.12-2.21) and positive attitude (aOR = 4.94, 95%CI = 1.19-20.44) to COVID-19 vaccination. Physicians had better KAPP scores than other medical professionals. HCWs working in departments with high-risk patients were more likely to have good knowledge (aOR = 1.28, 95%CI = 1.00-1.72). Positive attitude was also associated with being male (aOR = 2.97, 95%CI = 1.75-5.07) and having at least one non-communicable disease (aOR = 1.92, 95%CI = 1.14-3.23). Being male (aOR = 1.97, 95%CI = 1.35-2.88) and having more years of professional experience (aOR = 1.81, 95%CI = 1.29-2.52) were associated with good practice. <b>Conclusions</b>: Just over a third of HCWs in primary healthcare clinics had good knowledge of COVID-19 vaccination, while positive attitudes and good practices were low. Targeted interventions, particularly for HCWs with less professional experience working in rural settings, are needed to increase good practices and improve COVID-19 vaccination coverage in Tunisia.