Therapeutic Potentials of Cinnamomum Impressinervium Leaf Extracts: In Vitro Antidiabetic and Anti-Inflammatory Activities

Background: Cinnamomum impressinervium (Lauraceae), locally known as tejiya, is an underexplored plant with potential therapeutic significance. Traditional claims suggest its use in treating metabolic and inflammatory conditions, yet limited scientific vali-dation exists. Given the global burden of diabetes and chronic inflammation, identifying safe and affordable plant-derived alternatives remains a priority. Objective: This study evaluates the in vitro antidiabetic and anti-inflammatory activities of C. impressinervium leaf extracts using α-amylase inhibition and protein denaturation assays. Methods: Aqueous, methanolic, chloroform, and n-hexane extracts were prepared via hot percolation. Extracts were tested for α-amylase inhibition (100–500 μg/mL) using acarbose as a positive control, and for protein denaturation inhibition (10–100 μg/mL) using diclofenac as a reference. Each experiment was performed in triplicate, and results were expressed as mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test (p < 0.05). Results: Aqueous extracts exhibited the strongest α-amylase inhibition (40.64–77.67% across doses), followed by methanolic extracts (31.87–71.56%). In protein denaturation assays, aqueous (62.38%) and methanolic (61.07%) extracts demonstrated significant inhibition at 100 μg/mL compared to chloroform (22.62%) and n-hexane (17.17%). Positive controls showed higher inhibition values, validating assay reliability. Conclusion: Findings indicate that aqueous and methanolic extracts of C. impressinervium possess notable in vitro antidiabetic and anti-inflammatory properties, likely due to their phy-toconstituents. Further studies on phytochemical profiling, mechanism elucidation, and in vivo validation are warranted to develop standardized formulations