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Pro-inflammatory cytokines such as IL10, IL6, and TNFα are key players in the inflammatory response associated with acute lung injury. Excessive levels of these cytokines can worsen lung conditions, making their suppression a potential treatment strategy. Previous research suggests that pearl grass extract has the potential to alleviate inflammation. In the present study, we evaluated the effects of ethanol extract from pearl grass on the TNFα, IL-6, and IL-10 levels in mice with lung inflammation induced by lipopolysaccharide (LPS). Twenty-five mice were served as a normal group (received distilled water), a negative control group (administered LPS without the Extract), and groups that received LPS along with 750, 500, and 250 mg kg -1 BW pearl grass extract. The test preparation was given intragastrium for seven days, followed by intranasal LPS on day eight. Seventy-two hours after LPS exposure, BAL fluid was collected, and BAL fluid cytokine levels were measured using ELISA. Pearl grass extract 250 mg kg-1 BW remarkably reduced TNFα, and IL6 levels but did not affect IL10. These findings suggest that pearl grass extract could help alleviate acute lung inflammation by regulating pro-inflammatory cytokines, indicating a potential treatment approach to reduce disease severity