Race and Sex‐ specific Association of Sleepiness Scores and SIMOA assessed plasma AD biomarkers in Cognitively Normal Non‐Sleepy Older Adults

Abstract Background Excessive daytime sleepiness (EDS) is associated with poor cognitive performance and increased Alzheimer's Disease (AD) risk. However, the association between sleepiness scores in non‐ sleepy individuals and plasma AD biomarkers remains under‐explored. We examined the association between sleepiness scores and plasma AD biomarkers in a diverse sample of cognitively normal community dwelling non‐ sleepy older adults and studied for race and sex differences of this association. Method We conducted a cross‐sectional analysis of 186 cognitively normal, community‐ dwelling older adults (103 whites, 83 blacks) from various NYU studies on sleep, aging, and memory. Excessive daytime sleepiness (EDS) was assessed using the Epworth Sleepiness Scale (ESS). Plasma AD biomarkers (Aß‐42, Aß, p ‐tau, NfL, Total Tau, GFAP) were measured using SIMOA. Linear mixed effects models controlled for age, sex, race, cognitive status, and education, examining ESS association with plasma markers and interactions between ESS* race and ESS*sex . Result The study participants had an average of 72.7±6.5y, and 16.6± 2.2y of education. 68.8% of participants female, Mean ± SD for ESS was 4.2± 3.7 (≥ 11 suggest EDS), and 407.0 ± 85.6 minutes for total sleep time. Independently, sleepiness scores were not associated with the levels of any Plasma AD biomarkers. However, in the interaction model for GFAP levels, race ESS, and Race*ESS interaction were significant (ß[Race*ESS] = 9.67[2.99, 16.35]; p <0.05). With each unit increase in sleepiness scores, Blacks were more likely to have higher GFAP levels. Furthermore, Race only had an independent effect on pTau 181/Aß42 levels(ß[Race] =0.059 [‐0.117, ‐0.001] with Blacks having lower levels. Sex only had an independent effect on GFAP, Aß42/Aß40, and pTau181/Aß42, levels (ß[Sex]=27.55 [2.51, 52.58]; ß [Sex] = ‐0.06 [‐0.11, ‐0.01]; p < ) 0.05, respectively), with females having higher levels of GFAP, and Aß42/Aß40, but lower pTau181/Aß42 levels. Conclusion In this non‐sleepy, cognitively healthy diverse sample of community dwelling older adults, sleepiness scores were not associated with any Plasma AD biomarkers. However, Race‐ specific effects of this association were observed with markers of inflammation, suggesting that neuronal inflammation may be related to EDS symptoms with a notable effect among Blacks.