Quantitative reverse transcription PCR coupled with high-resolution melting for simultaneous detection and quantification of four isoforms of BCR::ABL1
The present multiplex RT-qPCR/HRM assay may help streamline clinical workflows, enhance diagnostic precision and offer a practical platform for studying CML clonal dynamics. It also holds promise for facilitating interlaboratory standardisation of non-p210 quantification.
Authors
- Ayman Mohamed
- Ben Legendre
- Kojo S J Elenitoba-Johnson
- Ting Zhou
- Heather Newell
Institutions
- Memorial Sloan Kettering Cancer Center (US)
- Precipio (United States) (US)
Publication Details
- Journal
- Journal of Clinical Pathology
- Published
- 2026-01-08
- DOI
- https://doi.org/10.1136/jcp-2025-210447
- Primary Topic
- Chronic Myeloid Leukemia Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00