Development of a Universal Forward Primer and Species-Specific Reverse Primers for Porcine, Canine, and Murine Gene Detection using SYBRGreen Multiplex Quantitative Polymerase Chain Reaction

The developed primers were able to differentiate animal species concentrations found in pharmaceuticals and cosmetics with good repeatability. However, porcine peaks in sample analysis were still low due to the low yield of DNA extraction using a food-grade DNA extraction kit Conclusion: These results suggest that the new primer combination, consisting of the universal forward primer and species-specific reverse primers, has the potential to serve as an alternative assay for differentiating canine, porcine, and murine DNA using multiplex intercalating dyebased qPCR.