Benchtop NMR signal enhancement of metabolites in urine extract using SABRE
Metabolites in a urine extract are signal-enhanced by SABRE hyperpolarization and detected using a benchtop NMR spectrometer. Quantification by standard addition is demonstrated for endogenic urinary nicotinamide (vitamin B3). Even higher sensitivity is achieved in an automated setup for multi-scan SABRE experiments. This hyperpolarization scheme is able to expedite biomarker detection and quantification, while maintaining low infrastructure requirements.
Authors
- Kerti Ausmees (ORCID: https://orcid.org/0000-0001-7680-1515)
- Simon Fleischer (ORCID: https://orcid.org/0000-0003-3554-8888)
- Jan Gerrit Korvink (ORCID: https://orcid.org/0000-0003-4354-7295)
- Indrek Reile (ORCID: https://orcid.org/0000-0003-3278-7947)
- Sören Lehmkuhl (ORCID: https://orcid.org/0000-0002-1321-7677)
- Neil MacKinnon
- Jing Yang
Institutions
- Karlsruhe Institute of Technology (DE)
- National Institute of Chemical Physics and Biophysics (EE)
Publication Details
- Journal
- Chemical Communications
- Published
- 2026-01-01
- DOI
- https://doi.org/10.1039/d5cc06186e
- Primary Topic
- Advanced NMR Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Keemilise ja Bioloogilise Füüsika Instituut
- Deutsche Forschungsgemeinschaft