Effect of the <scp>R80T</scp> mutation in the nicotinic acetylcholine receptor <scp> <i>β1</i> </scp> subunit on the susceptibility of <scp> <i>Frankliniella occidentalis</i> </scp> (Pergande) to neonicotinoids

We established the first functional heterologous expression system for F. occidentalis nAChRs, revealing that the β1 subunit R80T mutation significantly reduces neonicotinoid affinity. This clarifies the molecular interaction mechanism and supports proactive resistance monitoring and targeted insecticide development. © 2026 Society of Chemical Industry.