Investigation of gene stability in equine luteal tissue during mid-diestrus phase and early pregnancy - Research Article -

Commonly used reference genes (ACTB, GAPDH, B2M) for RT-qPCR normalization were amongst the least stably expressed genes in equine corpus luteum samples of pregnant and nonpregnant mares at days 11 and 13 of gestation. The most stably expressed putative reference genes using 3 different analysis modalities were SRP14, COX4I1, RPL13 and RPL4. Exploration of putative reference genes should be considered when investigating dynamic endocrine organs such as those used in reproductive studies.