Nano-Hydroxyapatite/β-Tricalcium Phosphate (n-HA/β-TCP) and Type 1 Collagen Block-Shaped Composite: In Vitro Analysis and Physicochemical Characterization

New nano-biomaterials for specific dentistry applications have been developed thanks to contributions from materials science. The present work aims to characterize the physicochemical properties of a composite nanomaterial scaffold in block form for maxillofacial bone regeneration applications. The scaffold was composed of block-shaped elements and consisted of a mixture of nano-hydroxyapatite, β-tricalcium phosphate, and type I collagen of bovine origin. Collagen I molecule is biodegradable, biocompatible, easily available, and a natural bone matrix component. The biomaterial was analyzed using a range of methods, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), chemical composition microanalysis, and X-Ray diffractometry (XRD). The wettability was measured. This was carried out by measuring the contact angle of a 0.9% NaCl solution on the surface. Differential scanning calorimetry (DSC) was used to measure the phase transformation temperatures. In the SEM and TEM analyses, it was possible to identify the layers of the materials and, with microanalysis, quantify their chemical composition. The XRD spectra showed the presence of nano-hydroxyapatite and ß-TCP. Wettability testing revealed that the material is highly hydrophilic, and BM-MSC culture analyses demonstrated that the biomaterial can promotes cell adhesion and interaction. The higher wettability is due to the higher density of the porous material observed in the SEM analysis. The results of the DSC testing showed that the sample analyzed undergoes endothermic transitions and transformation between 25 and 150 °C. The first phase transformation during heating occurs at 61.1 °C, which is above body temperature. The findings demonstrated that the composite was devoid of any contamination arising from manufacturing processes. It can be concluded that the n-HA/β-TCP and type 1 collagen are free of manufacturing contaminants. They also have high wettability, which increases the spreading of body fluids on the biomaterial’s surface and its interactions with cells and proteins. This makes them suitable for clinical application.