Histo-Morphometric Study of Teeth using the Petrographic Method: A Novel Technique

Abstract Purpose: Oral histology, which involves the study of tooth tissue, is crucial for clinical practice in dentistry. Additionally, a comprehensive understanding of the structure of dental hard tissue is highly significant for several dental specialties. Nevertheless, acquiring knowledge and comprehending the intricacies of dental anatomy can be arduous to visualize under microscopy given the complex structure and degree of calcification. The purpose of this research is to create a new technique for ground sections, making the experience enhance the quality and efficiency and create an aptitude for hands-on research and an enthusiasm for learning new scientific concepts, laying the framework for independent research in the future. Materials and Methods: About 20 deciduous and 20 permanent extracted teeth were subjected to petrographic sectioning using a Buehler petro-thin sectioning system and observed under a light microscope for histo-morphometric features. Results: The results of our study revealed that the new petrographic technique of tooth sectioning can be incorporated into the dental curriculum to simply and effectively research the pathophysiology and histology of tooth-hard tissue. Conclusion: This novel experience of creating ground sections can enhance and elicit theoretical knowledge and eliminate static observation and less manipulative opportunities. Reforms should be made to introduce the petrographic method in the dental curriculum and research and accept acquiring teeth ground sections may be made easier with our innovative cutting machine.