In-vitro antibacterial assessment of o deodorant roll - on formulated from Gatra Dourgandhyanashana Lepa with underarm swab samples

Introduction: Sweda (sweat), a bodily exudation linked to fat tissue, helps maintain skin moisture but excessive sweating causes bad odor and itching. In Ayurveda, various formulations are outlined to reduce deha dourgandhya (bad odor). One such remedy is the gatra dourgandhyanashana lepa, comprising vasa (Adhatoda vasica Nees.) and shankha (conch shell). In this study, deodorant the modified form of gatra dourgandhyanashana lepa was tested for antibacterial activity. Materials and methods: The bacterial load before and after application of deodorant was assessed by measuring the total viable count using in-vitro serial dilution of underarm swabs. The obtained results of before application (BA), after application 2hrs (AA2) and after application 4hrs (AA4) were subjected to statistical analysis. Results and Discussion: Descriptive statistics showed mean bacterial counts of 6188.89 ± 4088.534 (BA), 2557.78 ± 2021.273 (AA2), and 2166.67 ± 1161.895 (AA4). The Friedman test (p = 0.034) indicated a statistically significant reduction in microbial load in the study samples between at least two of the time points. Wilcoxon’s test revealed AA4 showed significant reduction (p = 0.002), but AA2 did not (p = 0.049), suggesting deodorant's effectiveness at 4 hours. Conclusion: The present study demonstrated a decrease in bacterial counts, with the effect persisting four hours after the application of the deodorant roll-on.