Field Susceptibility of Almond (Prunus dulcis) Cultivars to Red Leaf Blotch Caused by Polystigma amygdalinum in Apulia (Italy) and Influence of Environmental Conditions

<i>Polystigma amygdalinum</i> the causal agent of Red Leaf Blotch (RLB), is responsible for one of the most important foliar diseases affecting almond [<i>Prunus dulcis</i> (Miller) D.A. Webb] in the Mediterranean Basin and the Middle East. The study is aimed at improving knowledge on RLB epidemiology and the role of environmental conditions in disease development. Field monitoring was conducted from 2022 to 2025 in three almond orchards located in Apulia (southern Italy) and characterized by different microclimatic conditions. A total of 39 cultivars, including Apulian local germplasm and international cultivars ('Belona', 'Genco', 'Guara', 'Ferragnès', 'Filippo Ceo', 'Lauranne^® Avijor', 'Soleta', and 'Supernova'), were evaluated. Symptoms occurred from late spring to summer, resulting particularly severe on 'Guara' and 'Lauranne^® Avijor', whereas 'Belona', 'Ferragnès', 'Genco', and 'Supernova' exhibited the highest tolerance. To our knowledge, this is also the first report of RLB tolerance by 'Filippo Ceo', 'Ficarazza', 'Centopezze', and 'Rachele piccola' representing potential genetic resources for breeding programs. Moreover, these findings reinforced previous observations proving that RLB was less severe on medium-late and late cultivars. Disease incidence varied significantly among sites and years and was strongly associated with increased rainfall, higher relative humidity, and mild temperatures recorded in November, influencing disease occurrence in the following growing season. <i>P. amygdalinum</i> was consistently detected by qPCR in all RLB-affected tissues and, in some cases, from mixed early RLB + <i>Pseudomonas</i>-like symptoms. From some leaves with early RLB symptoms, <i>P. amygdalinum</i> was also successfully isolated in pure culture. Overall, our results provide clear evidence that <i>P. amygdalinum</i> is the sole fungal pathogen consistently associated with typical RLB symptoms in Apulia (southern Italy) and highlight important cultivar-dependent differences. Its frequent molecular detection in leaves showing atypical or mixed symptoms suggests unresolved epidemiological aspects requiring further investigation.