Chromosome-level genome assembly of Tadehagi triquetrum provides new insights into genome evolution and biosynthesis of tadehaginoside

• First high-quality level chromosome genome assembly of Tadehagi triquetrum provides a high-quality reference genome (627.33 Mb, contig N50 34.04 Mb) with exceptional completeness (BUSCO score: 99.2%). • Comparative genomics analysis reveals phylogenetic position and whole-genome duplication events, showing divergence from soybean ∼34.16 million years ago. • Identification and functional characterization of TtHCT gene elucidates key enzymatic step in tadehaginoside biosynthesis.