Validation of Chemical Inactivation Protocols for Henipavirus-Infected Tissue Samples

Biocontainment laboratories often have limited access to a range of instruments required for conducting standard assays on infected materials. Consequently, some of the protocols involving infected samples are conducted outside a biocontainment facility. To be compliant with regulatory requirements and minimize health and safety risks for scientific personnel, it is imperative to test procedures rigorously for safely removing infected samples from biocontainment areas. This study validated the chemical inactivation of Nipah virus (NiV), a representative member of the <i>Henipavirus</i> genus, in animal tissues and serum. Importantly, this work demonstrated successful NiV-spiking of non-human primate (NHP) tissues and their subsequent inactivation. This is important because NHP tissues contain unpredictable amounts of infectious virus. The primary objective was to establish standardized protocols that are compliant with regulations to permit safe retrieval of infected biological samples with high NiV infectious virus content from ABSL-4 laboratories for subsequent downstream processing under lower biocontainment conditions.