Progress in the detection of gut microbiota based on microfluidic technology

Abstract Gut microbiota, a complex microbial community in the human intestine, are vital for digestion, nutrient absorption, and immune regulation, maintaining gut micro‐ecological balance. Accurate detection of its composition, structure, and function is crucial for understanding the relationship between health and disease, early disease diagnosis, personalized treatment, and exploring disease mechanisms. Traditional detection methods such as culture and conventional polymerase chain reaction (PCR) have limitations. Culture methods, which rely on specific media, struggle with anaerobic and fastidious microbes and thus have long detection times. Conventional PCR is complex, has low throughput, and is prone to contamination, not meeting the need for efficient, accurate, and high‐throughput analysis. Microfluidic technology, manipulating small fluid volumes through microscale channels, offers new opportunities with advantages such as simple operation, low reagent consumption, and fast analysis. This review encompasses the microfluidic‐based detection methods of gut microbiota in recent years. It expounds on the principles of these methods and analyzes their achievements, advantages, and limitations in practical applications. The aim is to provide multi‐dimensional technical references for relevant research, thereby promoting the application of this technology in the research of gut microbiota and the management of gut diseases.