Dechlorination of mixed chlorinated organophosphate esters (V6 and TCEP) and associated reductive dehalogenase gene expression by <i>Dehalococcoides mccartyi</i>
Commercial V6, a chlorinated organophosphate esters mixture, is widely used in polyurethane foam and has been detected in various environmental matrices. This study is the first to elucidate the microbial transformation pathways and mechanisms of commercial V6. A mixed culture containing <i>Dehalococcoides mccartyi</i> was found to dechlorinate V6 and tris(2-chloroethyl) phosphate (TCEP) into phosphate de-esterification products, chloride ion, and ethene. Notably, two reductive dehalogenase genes were simultaneously transcribed and their corresponding enzymes co-expressed, indicating a key role of <i>D. mccartyi</i> in the natural attenuation of commercial V6 in the environment.
Authors
- Junhong Wu
- Jianzhong Song (ORCID: https://orcid.org/0000-0002-4467-4483)
- Yin Zhong (ORCID: https://orcid.org/0000-0003-1366-3324)
- Lihua Yang (ORCID: https://orcid.org/0000-0003-2595-3396)
- Dan Li (ORCID: https://orcid.org/0000-0002-4493-7562)
- Heli Wang (ORCID: https://orcid.org/0000-0003-4207-0413)
- Qiulin Yang
- Yirong Deng
- Ping'an Peng
- Sen Yang
Institutions
- Dongguan University of Technology (CN)
- Guangzhou Institute of Geochemistry (CN)
- Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (CN)
- Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality (CN)
- Guangdong Academy of Forestry (CN)
- Guangdong Provincial Academy of Environmental Science (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Applied and Environmental Microbiology
- Published
- 2026-01-06
- DOI
- https://doi.org/10.1128/aem.01990-25
- Primary Topic
- Microbial bioremediation and biosurfactants
- Type
- article
- Field-Weighted Citation Impact
- 0.00