From Functional Food to Therapeutic Prospect: Mechanistic Study of Gypenoside XVII in HeLa Cells

Cervical cancer remains a prominent cause of cancer-related mortality among women worldwide because of chronic infection with high-risk human papillomavirus (HPV) and disparate access to prevention and treatment. The current research evaluates the anticancer activity of Gypenoside XVII, a bioactive saponin of <i>Gynostemma pentaphyllum</i>, in HeLa cells as a model of cervical cancer. MTT, Annexin V-PI, and Hoechst 33342 assays showed dose-dependent growth inhibition with typical apoptotic morphology. Flow cytometry revealed G<sub>0</sub>/G<sub>1</sub> cell-cycle arrest, while pathway interrogation revealed participation of mitochondrial and death-receptor cascades, in agreement with caspase-9 and caspase-8 activation, respectively. Collectively, these findings position Gypenoside XVII as a natural-product bioactive with potential both as an anticancer lead and as a functional-food ingredient, deserving of further preclinical development.

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Publication Details

Journal
Molecules
Published
2026-01-08
DOI
https://doi.org/10.3390/molecules31020214
Primary Topic
Ginseng Biological Effects and Applications
Type
article
Field-Weighted Citation Impact
0.00
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article

From Functional Food to Therapeutic Prospect: Mechanistic Study of Gypenoside XVII in HeLa Cells

Shoaib Younas, Sayed Sajid Hussain, Feng Feng Wang, Weijie Li et al.
Molecules
Ginseng Biological Effects and Applications
article

From Functional Food to Therapeutic Prospect: Mechanistic Study of Gypenoside XVII in HeLa Cells

Shoaib Younas, Sayed Sajid Hussain, Feng Feng Wang, Weijie Li, Muhammad Maisam
article en

Abstract

Cervical cancer remains a prominent cause of cancer-related mortality among women worldwide because of chronic infection with high-risk human papillomavirus (HPV) and disparate access to prevention and treatment. The current research evaluates the anticancer activity of Gypenoside XVII, a bioactive saponin of <i>Gynostemma pentaphyllum</i>, in HeLa cells as a model of cervical cancer. MTT, Annexin V-PI, and Hoechst 33342 assays showed dose-dependent growth inhibition with typical apoptotic morphology. Flow cytometry revealed G<sub>0</sub>/G<sub>1</sub> cell-cycle arrest, while pathway interrogation revealed participation of mitochondrial and death-receptor cascades, in agreement with caspase-9 and caspase-8 activation, respectively. Collectively, these findings position Gypenoside XVII as a natural-product bioactive with potential both as an anticancer lead and as a functional-food ingredient, deserving of further preclinical development.

MoleculesVol. 31(2)
University of Shanghai for Science and Technology (CN), Hefei University of Technology (CN)
Good health and well-being
Openalex Percentile: Top 94%
Ginseng Biological Effects and Applications
DOIOpen Access PDFOpenAlex
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From Functional Food to Therapeutic Prospect: Mechanistic Study of Gypenoside XVII in HeLa Cells — Shoaib Younas, Sayed Sajid Hussain, et al. · Molecules (2026) | TGRS Research Map | TGRS