Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust
This method is operationally simple, non-toxic, and biocompatible, achieving high total recovery of MTB cells while maintaining a high proportion of viable among the recovered bacteria. It offers substantial potential for environmental monitoring and downstream sequencing analysis, aiding in environmental risk assessment and management of clustered tuberculosis outbreaks.
Authors
- Ning Wei (ORCID: https://orcid.org/0000-0002-6069-0483)
- Fang Peng (ORCID: https://orcid.org/0000-0003-3864-2597)
- 彬 今田 (ORCID: https://orcid.org/0000-0003-4546-3781)
- Ming-Zhong Xu
- Feng Huang (ORCID: https://orcid.org/0000-0001-6215-8223)
- Lan Wen (ORCID: https://orcid.org/0000-0002-9907-3722)
- Jingjing Huang (ORCID: https://orcid.org/0000-0002-3009-8681)
- Bin Zhang
- Hui-ping Zhang
- Miao-miao Wang
Institutions
- Central South University (CN)
- Hunan Provincial Center for Disease Control and Prevention (CN)
- Guangzhou Blood Center (CN)
Publication Details
- Journal
- BMC Infectious Diseases
- Published
- 2026-01-07
- DOI
- https://doi.org/10.1186/s12879-025-12332-0
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00