EFFECTS OF SUB-ACUTE ADMINISTRATION OF FERMENTED ELAEIS GUINEENSIS SAP (PALM WINE) ON THE CEREBELLUM OF ADULT WISTAR RATS

Abstract: Background: Local storage and preservative practices of tapped oil palm (Elaeis guineensis) sap notably called “palm wine” do not prevent its fermentation and thus increase its alcoholic contents. Yet, palm wine is still chronically consumed even to toxic dimensions. Aim: This study aims at evaluating the effects of the sub-acute administration of fermented Elaeis guineensis Sap (palm wine) on the cerebellum of the brain using adult wistar rats. Methodology: Freshly tapped palm wine (Elaeis guineensis Sap) was diluted with 50% clean tap water, and allowed to ferment at room temperature for 24 hours before being administered to the rats. Twenty five (25) healthy male wistar rats (average weight = 200g) were divided into five groups (n=5). Group A served as the normal controls and received only distilled water daily. Groups B, C, D, and E were received increasing volumes of fermented palm wine via oral routes in increasing progressions (1ml, 2ml, 4ml, and 8ml respectively). The experimental procedure lasted for 14 days. 24 hours after their last administration, the rats were sacrificed under ketamine (100mg/ml) as anesthesia. Each brain masse was carefully harvested, and each cerebellum was isolated for histological analysis and stained with Cresyl Fast Violet and also Hematoxylin and Eosin. Results: Treated groups showed progressive dose-related injuries evidenced by fatty change, intracytoplasmic inclusions, vacuolation of purkinje cells, focal areas of necrosis and focal areas of microcystic spaces relative to the untreated control group. Conclusion: Treatment with increasing doses of fermented Elaeis guineensis Sap (palm wine) demonstrated clear dose-dependent deleterious effectson the histology of the cerebellum of adult wistar rats. Keywords: Elaeis guineensis, Fermentation, Alcohol, Cerebellum. Title: EFFECTS OF SUB-ACUTE ADMINISTRATION OF FERMENTED ELAEIS GUINEENSIS SAP (PALM WINE) ON THE CEREBELLUM OF ADULT WISTAR RATS Author: Christian Chiemeka Ozor, Chike Ikechukwu Patrick Anibeze, Godson Emeka Anyanwu International Journal of Recent Research in Life Sciences (IJRRLS) ISSN 2349-7823 Vol. 12, Issue 4, October 2025 - December 2025 Page No: 52-58 Paper Publications Website: www.paperpublications.org Published Date: 31-December-2025 DOI: https://doi.org/10.5281/zenodo.18110009 Paper Download Link (Source) https://www.paperpublications.org/upload/book/EFFECTS%20OF%20SUB-ACUTE%20ADMINISTRATION-31122025-7.pdf