Optimizing Post-Transplantation Detection of Subcutaneously Transplanted Islets Using Dithizone Staining

Dithizone staining is a rapid, cost-effective approach to identify subcutaneous islet grafts and enables downstream analyses, addressing a significant limitation in islet transplantation research. When combined with standardized pre-transplant islet quantification, this approach provides a cohesive and reproducible framework for evaluating subcutaneous islet transplantation. Future studies could complement these results by assessing the potential effects of dithizone exposure and exploring its applicability across other transplantation sites and imaging modalities.

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Publication Details

Journal
bioRxiv (Cold Spring Harbor Laboratory)
Published
2026-01-07
DOI
https://doi.org/10.64898/2026.01.06.697969
Primary Topic
Pancreatic function and diabetes
Type
article
Field-Weighted Citation Impact
0.00

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article

Optimizing Post-Transplantation Detection of Subcutaneously Transplanted Islets Using Dithizone Staining

Matthew Ishahak, James T. Lu, Marlie M. Maestas, Jeffrey R. Millman
bioRxiv (Cold Spring Harbor Laboratory)
Pancreatic function and diabetes
article

Optimizing Post-Transplantation Detection of Subcutaneously Transplanted Islets Using Dithizone Staining

Matthew Ishahak, James T. Lu, Marlie M. Maestas, Jeffrey R. Millman
article en

Abstract

Dithizone staining is a rapid, cost-effective approach to identify subcutaneous islet grafts and enables downstream analyses, addressing a significant limitation in islet transplantation research. When combined with standardized pre-transplant islet quantification, this approach provides a cohesive and reproducible framework for evaluating subcutaneous islet transplantation. Future studies could complement these results by assessing the potential effects of dithizone exposure and exploring its applicability across other transplantation sites and imaging modalities.

bioRxiv (Cold Spring Harbor Laboratory)
Washington University in St. Louis (US)
Edward Mallinckrodt, Jr. Foundation, National Institutes of Health
No poverty
Openalex Percentile: Top 95%
Pancreatic function and diabetes
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