Optimizing Post-Transplantation Detection of Subcutaneously Transplanted Islets Using Dithizone Staining
Dithizone staining is a rapid, cost-effective approach to identify subcutaneous islet grafts and enables downstream analyses, addressing a significant limitation in islet transplantation research. When combined with standardized pre-transplant islet quantification, this approach provides a cohesive and reproducible framework for evaluating subcutaneous islet transplantation. Future studies could complement these results by assessing the potential effects of dithizone exposure and exploring its applicability across other transplantation sites and imaging modalities.
Authors
- Matthew Ishahak (ORCID: https://orcid.org/0000-0002-7494-6048)
- James T. Lu (ORCID: https://orcid.org/0000-0002-1873-7049)
- Marlie M. Maestas (ORCID: https://orcid.org/0000-0003-4396-9770)
- Jeffrey R. Millman
Institutions
- Washington University in St. Louis (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-01-07
- DOI
- https://doi.org/10.64898/2026.01.06.697969
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Edward Mallinckrodt, Jr. Foundation
- National Institutes of Health