To stain or not to stain: unraveling potential bacterial contamination in germ-free husbandry using bacterial viability staining
Altogether, detected bacterial structures were proven to be non-viable and therefore should not be interpreted as isolator contaminants. Thus, in our hands, with the herein described report of suspected contamination, we prove that bacterial viability staining served as a highly valuable screening tool, enhancing diagnostic quality of the HM of GF colonies.
Authors
- Stephanie Buchheister (ORCID: https://orcid.org/0000-0002-0589-5857)
- Anna Smoczek
- Tim Scheele
- M Basić
- Silvia Bolsega
- André Bleich
- Susan Rösel-Birk
Institutions
- Medizinische Hochschule Hannover (DE)
- Institute of Laboratory Animal Science (CN)
Publication Details
- Journal
- Journal of Applied Microbiology
- Published
- 2026-01-01
- DOI
- https://doi.org/10.1093/jambio/lxag001
- Primary Topic
- Animal testing and alternatives
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Joint Programming Initiative A healthy diet for a healthy life
- Deutsche Forschungsgemeinschaft
- Bundesministerium für Bildung und Forschung
- Medizinischen Hochschule Hannover