Clinical actionability in gliomas revealed by real-world next-generation sequencing: a multicentric study
Treatment options for patients with gliomas remain limited, and prognosis is generally poor. While next-generation sequencing (NGS) is increasingly used to stratify glioma patients and guide therapy, its implementation in routine clinical practice remains variable. We conducted a multicenter retrospective study across seven Spanish hospitals to evaluate the clinical utility of NGS in glioma management, focusing on its impact on diagnosis and treatment selection based on the ESMO Scale for Clinical Actionability of Molecular Targets (ESCAT). A total of 541 glioma patients diagnosed between 2018 and 2022 were included; 76% had glioblastomas and 24% other glioma subtypes. Among glioblastoma patients, 9% harbored ESCAT tier 1/2 alterations and 74% tier 3/4. Molecularly matched therapy was administered in 10.2% of glioblastoma cases. Objective responses were observed in 17.6% of glioblastoma and 33% of non-glioblastoma patients with ESCAT tier 1/2 alterations. Patients with tier 1/2 alterations experienced significantly longer progression-free survival compared to those with tier 3/4. These findings support genomic profiling of gliomas in research centers to expand therapeutic options in molecularly guided clinical trials.
Authors
- Marta Doménech (ORCID: https://orcid.org/0000-0003-4827-9170)
- Julia Lostes (ORCID: https://orcid.org/0000-0002-0371-7598)
- Diego Gómez-Puerto
- Maria Alba Sorolla (ORCID: https://orcid.org/0000-0002-2180-0408)
- Maria Castro-Henriques
- Maria Margarida Correia Alves Vieito
- Elena Martinez-Saez
- C. Balana
- Oriol Mirallas (ORCID: https://orcid.org/0000-0002-8492-5195)
- J. Yaringaño (ORCID: https://orcid.org/0009-0004-4275-1563)
- Teresa Gorría
- Joan Carles
- Óscar Gallego (ORCID: https://orcid.org/0000-0001-5665-0967)
- M. Martinez-Garcia
- Álvaro Martinez-Monino
- Ainhoa Holgado (ORCID: https://orcid.org/0000-0002-7469-1970)
- Estela Pineda
- María Ángeles Vaz
- Juan Manuel Sepúlveda
- Rodrigo Dienstmann
- G. Velilla
- Daniel López-Valbuena
- Antonio Di Muzio
- Fiorella Ruiz-Pace
Institutions
- Universitat de Vic - Universitat Central de Catalunya (ES)
- Hebron University (PS)
- Humanitas University (IT)
- Instituto Cajal (ES)
- Hospital de Sant Pau (ES)
- Research Institute Hospital 12 de Octubre (ES)
- Hospital Clínic de Barcelona (ES)
- Instituto Ramón y Cajal de Investigación Sanitaria (ES)
- Vall d'Hebron Hospital Universitari (ES)
- Hospital Del Mar (ES)
- Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol (ES)
- Consorci Institut D'Investigacions Biomediques August Pi I Sunyer (ES)
- Vall d'Hebron Institute of Oncology (ES)
Publication Details
- Journal
- npj Precision Oncology
- Published
- 2026-01-06
- DOI
- https://doi.org/10.1038/s41698-025-01247-3
- Citations
- 1
- Primary Topic
- Glioma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 36.93