Antihemolytic and Thrombolytic Potential of <i>Ocimum basilicum</i> Seed Extract, Bioactive Compounds, and Docking With VanA Ligase in Vancomycin‐Resistant Staphylococci

<i>Ocimum basilicum</i> is an important alternative source to explore diverse anti-infective compounds. In the present study, aqueous seed extract of <i>O. basilicum</i> was used to identify bioactive compounds with antihemolytic, thrombolytic, antivancomycin-resistant <i>Staphylococcus aureus</i> and antivancomycin-resistant <i>S. epidermidis</i> activity. Anti-VRSA and anti-VRSE activity of <i>O. basilicum</i> seed aqueous extract was evaluated by the well diffusion assay. Hemolytic and thrombolytic activities were performed using a 96-well plate. Phytochemical identification was done by GC-MS. ADMET and docking analyses with VanA ligase of VRSA and VRSE were also performed. The aqueous extract showed antibacterial activity against VRSA (12 ± 0.35 mm) and VRSE (13 ± 0.11 mm) isolates. The <i>O. basilicum</i> showed significantly less hemolysis (3.7 ± 0.24%, <i>p</i> < 0.00001) of red blood cells, reflecting low cytotoxicity as compared to the control (98 ± 0.44%). The <i>O. basilicum</i> seed extract exhibited significant thrombolytic activity (4.33 ± 0.2%, <i>p</i> < 0.000429) as compared to the negative control (2 ± 0.34%). Among 23 identified compounds on GC-MS, eight were reported for the first time in <i>O. basilicum</i> aqueous seed extract and processed for molecular docking. After favorable water solubility, pharmacokinetics, medicinal chemistry, and drug likeness, only two compounds, d-glucopyranoside, 2,3,4,6-di-O-(ethylboranediyl)-1-O-methyl and 4(3,4-dihydroxy-2-oxo-butylamino) benzonitrile, were processed for molecular docking. The first one formed three hydrogen bonds with Leu-259, Ser-127, and His-49 residues of the VanA ligase. The second one formed two hydrogen bonds with Ser-161 and Val-160 residues of the VanA ligase. d-Glucopyranoside, 2,3,4,6-di-O-(ethylboranediyl)-1-O-methyl and 4(3,4-dihydroxy-2-oxo-butylamino) benzonitrile. The <i>O. basilicum</i> seed extract has potential bioactivity, and the identified compounds are novel putative VanA ligase inhibitors. Further characterization of the bioactive compounds would help to explore therapeutic targets against VRSA and VRSE.