Determination of the binding affinity between osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) utilizing Gaussia luciferase in living cells
This bioluminescence-based method provides a simple and accessible platform for evaluating interactions between soluble and membrane-bound proteins in living cells, with potential application in the development of drugs targeting the RANKL-RANK-OPG axis.
Authors
- Mamoru Matsubara (ORCID: https://orcid.org/0000-0002-8893-0485)
- Shinji Fukuda (ORCID: https://orcid.org/0000-0002-6920-1818)
- Takuma Sato (ORCID: https://orcid.org/0000-0002-5555-7709)
- Ken Miyazawa (ORCID: https://orcid.org/0000-0001-9211-1020)
- Yudai Izumi (ORCID: https://orcid.org/0000-0003-4524-9590)
- Nishida-Fukuda Hisayo
- Yuto Shibata
- Takahiro Suzuki
- Hironao Nakayama
- Satoru Yokawa
- Reina Suzuki
- Tadahide Furuno
Institutions
- Aichi Gakuin University (JP)
- Gifu University of Medical Science (JP)
- Hiroshima International University (JP)
- Institute for Molecular Science (JP)
Publication Details
- Journal
- Journal of Oral Biosciences
- Published
- 2026-01-08
- DOI
- https://doi.org/10.1016/j.job.2025.100727
- Primary Topic
- Bone Metabolism and Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00