Direct evidence for poison use on microlithic arrowheads in Southern Africa at 60,000 years ago

Poisoned weapons are a hallmark of advanced hunter-gatherer technology. Through targeted microchemical and biomolecular analyses, we identified traces of toxic plant alkaloids on backed microliths from Umhlatuzana Rock Shelter in KwaZulu-Natal, South Africa, excavated from a level dated to 60,000 years ago. The alkaloids buphandrine and epibuphanisine only originate from Amaryllidaceae indigenous to southern Africa. The most likely source is <i>Boophone disticha</i> (L.f.) Herb. bulb exudate, also associated with historically documented arrow poisons. To our knowledge, we present the first direct evidence for the application of this plant-based poison on the tips of Pleistocene hunting weapons. The discovery highlights the complexity of subsistence strategies and cognition in southern Africa since the mid-Pleistocene.