Possible Hepatoprotective Effects of Iraqi Grape Seeds Oil and Metformin on CCl4-Induced Rat Model of Liver Fibrosis

Objective: This study was performed to evaluate the synergistic antioxidative properties of grape seed oil and metformin in treating liver cirrhosis induced by carbon tetrachloride. Materials and Methods: This in vivo study included 40 adult rats categorized into four groups of eight rats per group. The experiment lasted for four weeks. Before the commencement of treatment, liver damage was induced in all rat groups involved in the study, except the negative control groups, by administering a single dose of CCL4. The control-positive group left without undergoing any treatment. Group Three received treatments of 0.2 ml/kg body weight of grape seed oil. Group four received a daily dosage of 150 mg/kg body weight of metformin. Group five was administered 150 mg/kg body weight of metformin daily, in addition to 0.2 ml/kg body weight of grape seed oil.Results: The study's findings on oxidative stress revealed that liver-injured animals treated with grape seed oil and metformin showed a significant reduction in malonaldehyde levels compared to group two, with no significant differences observed with groups three and four. Furthermore, regarding antioxidant activity, liver-injured animals that were treated with grape seed oil and metformin showed a significant increase in catalase and glutathione levels compared to group two, with no significant differences observed with groups three and four. Conclusions: This investigation's biochemical and histological findings demonstrate that combining grape oil and metformin has a greater hepatoprotective impact than either treatment alone, suggesting a possible synergistic interaction and offering an attractive hepatoprotective option.