Development of a Nano-Real-Time Polymerase Chain Reaction (RT-PCR) Kit for Detection and Genotyping of High-Risk Human Papillomavirus (HPV) Strains Using Dedicated TaqMan Probes

Incorporating AuNPs in RT-PCR significantly improved fluorescence signal detection, primer binding efficiency, and polymerase activity, enhancing sensitivity and specificity. Given its high diagnostic accuracy, this assay represents a promising tool for the early and reliable detection of HPV, with potential applications in clinical diagnostics and epidemiological surveillance.